70%（体积比）乙醇溶液：量取700 mL乙醇，加水稀释至1000 mL，混匀备用。

SelectCore HLB-P 200mg/6mL固相萃取柱

取上述步骤2中制备好的待净化液3 mL上样至固相萃取柱上，弃去前1 mL流出液，收集剩下流出液，吹干固相萃取柱，并收集流出液，混匀，供后续高效液相色谱法测定分析用。

Column: ChromCore C18, 5μm, 4.6×250mm

Mobile Phase:  A) 水

                         B) 乙腈

Flow rate: 1.0 mL/min

Temperature: 35 °C

Injection: 10 μL

Detection: UV 270 nm

Gradient:     t (min)         %A        %B

                     0.01            76         24

                    25.00           74         26

                    34.00           74         26

                    34.10           30         70

                    39.00           30         70

                    40.00           76         24

                    46.00           76         24